Aim We aimed to develop and physicochemically/biologically evaluate semisolid formulations containing gold nanoparticles loaded with betulinic acid (BA). Materials and methods Betulinic acid-conjugated gold nanoparticles were incorporated in three types of semisolid pharmaceutical formulations: nanoemulsion, oleogel and hydrogel. Each nanoformulation was prepared as blank, with AuNP and with AuNP+BA, respectively, and was physicochemically analyzed through