Aim The study focused on the development and assessment of semisolid formulations with betulin-conjugated gold nanoparticles. Materials and methods Three semisolid formulations containing betulin (Bet) and gold nanoparticles (AuNP) were developed, namely: 1) nanoemulsion, 2) oleogel and 3) chitosan-based hydrogel. Physico-chemical analysis of all nanoformulations (blank, with AuNP and with AuNP+Bet, respectively) was conducted through